rabbit anti mouse caspase3 antibody  (R&D Systems)

Journal: bioRxiv

Article Title: Nup358 Sustains Intestinal Epithelial Homeostasis by Preventing Dvl1 Condensate Formation to Restrain Wnt Signaling

doi: 10.64898/2026.03.25.714063

Figure Lengend Snippet: a. Quantification of body weight in Nup358 fl/fl and Nup358 fl/fl CreER T 2 mice treated with tamoxifen. Data are expressed as mean ± SD. ****p ≤ 0.0001. b. Small intestine length in Nup358 fl/fl and Nup358 fl/fl CreER T2 mice treated with tamoxifen. Data are expressed as mean ± SD. *p ≤ 0.05. c. Duodenum cross-sectional area in Nup358 fl/fl and Nup358 fl/fl CreER T2 mice treated with tamoxifen. Data are expressed as mean ± SD. **p ≤ 0.01. d. Representative images of hematoxylin and eosin stained small intestine section from Nup358 fl/fl and Nup358 fl/fl CreER T 2 mice treated with tamoxifen. Color boxes marked the zoom-in regions of crypt and villi. e. Immunofluorescence staining for Nup358 in small intestinal epithelium from Nup358 fl/fl and Nup358 fl/fl CreER T 2 mice treated with tamoxifen. f. Immunofluorescence staining for CD44, Ki67, and activated caspase 3 in small intestinal epithelium from Nup358 fl/fl and Nup358 fl/fl CreER T 2 mice treated with tamoxifen. Bottom: Quantification of immunofluorescence intensity per area for CD44, Ki67, and activated caspase 3.

Article Snippet: Antibodies used for immunofluorescence and immunoblotting are as follows: anti-Ki67 (Biolegend, #151206), anti-Nup358/RanBP2 (Bethyl Laboratories, A301-796A), anti-Nup358 (Santa Cruz Biotechnology, sc74518), anti-CD44 (Biolegend, #103012), Anti-activated Caspase 3 (CST #9664). anti-Olfm4 (CST # 39141), anti-TCF-4/TCF7L2 (CST, #2569), anti-Dvl1 (Santa Cruz, #sc8025), anti-Axin1 (CST, #2087), anti-α-Tubulin (CST, #3873), anti-ADP-Ribosylation (CST, #891990), anti-ADP-Ribosylation (CST, #89190), anti-HA (CST, #3724), anti-Histone 3 (CST # 9715), anti-Ubc9 (Abcam, ab33044), anti-Nup98 (Cell Signaling Technology, 2598P), anti-GAPDH (CST, #5174), and anti-GFP (Abcam, #1218).

Techniques: Staining, Immunofluorescence

Journal: Frontiers in Immunology

Article Title: Femoral fracture leads to moderate pulmonary organ damage in aged mice and induces immune alterations

doi: 10.3389/fimmu.2026.1763857

Figure Lengend Snippet: Lung damage in young and aged mice after femur fracture. Young (17–26 weeks) and aged (64–72 weeks) mice received a unilateral femur fracture or sham surgery. Twenty-four hours post-surgery, mice were euthanized, and sampling was performed. (A) Representative images of hematoxylin-eosin (HE) stained lung sections in a 40× magnification. (B) Concentration of total protein in bronchoalveolar lavage (BAL) fluid. (C) Representative images in a 40× magnification and (D) quantitative analysis of active caspase-3 expression in the lung. Data are presented as mean ± standard error of the mean; n = 6 per group. * = p < 0.05.

Article Snippet: Afterwards, the slides were blocked with blocking solution (5% goat serum, 0.05% TritonX, and 0.05% Tween20 in 1 × PBS) for 20 min at RT and incubated with the following primary antibodies for one hour at RT: rabbit anti-mouse C-X-C motif chemokine (CXCL)1 (1:300; #ab269939; Abcam Inc, Toronto, ON, Canada), rabbit anti-mouse advanced glycation end-products (RAGE) (1:100, #ab3611, Abcam Inc, Toronto, ON, Canada), rabbit anti-mouse neutrophil elastase (NE) (1:200; #bs-6982R; Bioss, USA), and rabbit anti-mouse active caspase-3 (Asp175) (1:300; #9661; Cell Signaling Technology, USA).

Techniques: Sampling, Staining, Concentration Assay, Expressing

Journal: Investigative Ophthalmology & Visual Science

Article Title: Morpho-Functional Characterization and miRNA Profiling of the Retina in the 5xFAD Murine Model of Alzheimer's Disease

doi: 10.1167/iovs.67.2.31

Figure Lengend Snippet: Evaluation of apoptosis in WT and in 5xFAD mouse retinas at 3, 6, and 9 months of age. ( A – D ) Representative images of retinal cross-sections immunolabeled for active caspase 3 with DAPI counterstain. The immunostaining patterns in WT retinas were similar at all ages, therefore, only one representative image of a WT retina is represented. Scale bar = 30 µm. ( E , F ) Representative Western blots and densitometric analysis of Bax ( E ), Bcl-2 ( F ), and relative quantification of Bax/Bcl-2 ratio ( G ). The levels of Bax and Bcl-2 were normalized to β-actin as well as to control (WT at 3 months of age). Two-way ANOVA followed by Bonferroni's post hoc test ( n = 6). ** P < 0.01 and **** P < 0.0001 versus age-matched WT.

Article Snippet: Rabbit monoclonal antibody anti-active caspase 3 , Cell Signaling , 9664S , 1:100.

Techniques: Immunolabeling, Immunostaining, Western Blot, Quantitative Proteomics, Control

Journal: Stem Cell Reports

Article Title: Histone methyltransferase Setd8 preserves chromatin accessibility to safeguard retinal progenitor cell identity during development

doi: 10.1016/j.stemcr.2025.102789

Figure Lengend Snippet: Setd8 deficiency reduces RPC proliferation and promotes apoptosis (A and D) Representative retinal section images of YFP (green) and EdU (magenta) staining at P0 (A) and P2 (D) from Ctrl (left) and Setd8 cKO (right) mice. Scale bars, 50 μm. (B and E) Quantification of the number of YFP+ (left graph) or EdU+ (right graph) cells at P0 (B) and P2 (E) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (C and F) Proportion of YFP+ EdU+ (double-positive) cells among YFP+ cells at P0 (C) and P2 (F) ( n = 15 from 5 retinas). ∗∗ p < 0.01. (G) Representative retinal section images of γH2AX (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (H) Quantification of the number of γH2AX + cells at P0 and P2 ( n = 15 from 5 retinas). (I) Representative retinal section images of YFP (green) and active caspase-3 (magenta) staining at P0 (left) and P2 (right) from Ctrl (upper) and Setd8 cKO (lower) mice. Scale bars, 50 μm. (J) Quantification of active caspase-3+ cells at P0 and P2 ( n = 15 from 5 retinas).

Article Snippet: The following primary antibodies were used: chicken anti-Nestin (1:500, NES, Aves Labs); goat anti-Sox2 (1:500, AF2018, R&D Systems); rabbit anti-active caspase 3 (1:500, AF-835, R&D Systems); chick anti-GFP (1:500, GFP-1010, Aves Labs); sheep anti-Chx10 (1:100, X1180P, Exalpha); rabbit anti-H4K20me1 (1:500, ab9051, Abcam); rabbit anti-S-opsin (1:500, AB5407, Millipore); rabbit anti-M-opsin (1:200, AB5405, Millipore); mouse anti-Brn3a (1:100, MAB1585, Millipore); mouse anti-Calbindin (1:500, CB300, Swant); goat anti-Otx2 (1:100, BAF1979, R&D Systems); mouse anti-Glutamine synthetase (1:500, 610517, BD Biosciences); rabbit anti-Pax6 (1:500, 901301, BioLegend); rabbit anti-γH2AX (1:500, ab2893, Abcam); and rat anti-BrdU (1:500, ab-6326, Abcam).

Techniques: Staining